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glutathione stransferase conjugated rhotekin rbd beads  (Cytoskeleton Inc)


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    Structured Review

    Cytoskeleton Inc glutathione stransferase conjugated rhotekin rbd beads
    Glutathione Stransferase Conjugated Rhotekin Rbd Beads, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 94/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/binding+activity/Rhotekin-RBD+beads+-+binds+active+Rho+proteins/pm42192125-442-16-21
    Average 94 stars, based on 69 article reviews
    glutathione stransferase conjugated rhotekin rbd beads - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Immunofluorescence:

    Article Title: Bone marrow mesenchymal stem cell-derived exosomes improve pyroptosis and mitochondrial integrity through miR-515-5p-mediated TLR4/NLRP3/GSDMD axis in rheumatoid arthritis
    Article Snippet: .. A Immunofluorescence analysis of RA-FLSs internalization of BMSCs-Exo; B Ach E activity assay to quantify BMSCs-Exo release; C – D Transwell assays to evaluate migration and invasion of RA-FLSs; E – F WB analysis showing representative images and quantification of ECM proteins collagen I and fibronectin; G Immunofluorescence staining of actin cytoskeleton in RA-FLSs. ..

    Article Title: Bone marrow mesenchymal stem cell-derived exosomes improve pyroptosis and mitochondrial integrity through miR-515-5p-mediated TLR4/NLRP3/GSDMD axis in rheumatoid arthritis.
    Article Snippet: .. A Immunofluorescence analysis of RA-FLSs internalization of BMSCs-Exo; B Ach E activity assay to quantify BMSCs-Exo release; C–D Transwell assays to evaluate migration and invasion of RA-FLSs; E–F WB analysis showing representative images and quantification of ECM proteins collagen I and fibronectin; G Immunofluorescence staining of actin cytoskeleton in RA-FLSs. ..

    Activity Assay:

    Article Title: Bone marrow mesenchymal stem cell-derived exosomes improve pyroptosis and mitochondrial integrity through miR-515-5p-mediated TLR4/NLRP3/GSDMD axis in rheumatoid arthritis
    Article Snippet: .. A Immunofluorescence analysis of RA-FLSs internalization of BMSCs-Exo; B Ach E activity assay to quantify BMSCs-Exo release; C – D Transwell assays to evaluate migration and invasion of RA-FLSs; E – F WB analysis showing representative images and quantification of ECM proteins collagen I and fibronectin; G Immunofluorescence staining of actin cytoskeleton in RA-FLSs. ..

    Article Title: Bone marrow mesenchymal stem cell-derived exosomes improve pyroptosis and mitochondrial integrity through miR-515-5p-mediated TLR4/NLRP3/GSDMD axis in rheumatoid arthritis.
    Article Snippet: .. A Immunofluorescence analysis of RA-FLSs internalization of BMSCs-Exo; B Ach E activity assay to quantify BMSCs-Exo release; C–D Transwell assays to evaluate migration and invasion of RA-FLSs; E–F WB analysis showing representative images and quantification of ECM proteins collagen I and fibronectin; G Immunofluorescence staining of actin cytoskeleton in RA-FLSs. ..

    Article Title: N-terminal phosphorylation inhibits Arabidopsis katanin and affects vegetative and reproductive development in opposite ways
    Article Snippet: Data were fit to a one-site saturation binding model, Y = B max *X / (K d + X), in GraphPad Prism. .. The ATPase activity of 25 nM of KTN1, DDD, or AAA in the presence of either 25nM or 250nM of taxol-stabilized microtubules was evaluated using a commercially available ATPase ELIPA Kit (Cytoskeleton #BK051) according to the manufacturer’s instructions. ..

    Article Title:
    Article Snippet: 1 ATP1B1 1 3.65 Plasma membrane Transport ATPase activity ATPase TM; Yes Yes gi49574534 HAVCR2 NP_116171.3 Hepatitis A virus cellular receptor 2 8 25.91 Plasma membrane Immune response Receptor activity Cell surface receptor IG; TM; SP; Yes Yes gi49574537 GCSH NP_004474.2 Glycine cleavage system H protein 1 11.56 Mitochondrion Metabolism Energy pathways Transferase activity Enzyme: Transferas e SP; Yes Yes gi50053795 EIF4B NP_001408.2 Eukaryotic translation initiation factor 4B 1 1.47 Cytoplasm Protein metabolism Translation regulator activity Translation regulatory protein RRM; CC; Yes Yes gi50234886 ZDHHC23 NP_775841.2 Zinc finger DHHC domain containing 23 1 2.45 Plasma membrane Cell communica tion Signal transductio n Molecular function unknown Unclassifie d TM; Yes No gi50301238 GSR NP_000628.2 Glutathione reductase 2 5.94 Mitochondrion Metabolism Energy pathways Catalytic activity Enzyme: Reductase SP; No Yes gi5031571 ACTR2 NP_005713.1 ARP2 actin-related protein 2 homolog 2 6.60 Actin cytoskeleton Cell motility Cell growth and/or maintenanc e Structural constituent of cytoskeleto n Cytoskelet al protein ACTIN; Yes Yes Protein accession Gene Symbol RefSeq accession Protein Description Unique number of peptides Sequence coverage Cellular component Biological process Molecular function Molecule class Architecture Replicate 1 Replicate 2 Supplemental Table 3. .. A Complete list of proteins identified from this study gi5031573 ACTR3 NP_005712.1 Actin related protein 3 5 20.57 Cytoplasm Cell growth and/or maintenanc e Structural constituent of cytoskeleto n Cytoskelet al protein ACTIN; Yes Yes gi5031587 APC2 NP_005874.1 Adenomatosis polyposis coli 2 1 0.26 Nucleus Cell communica tion Signal transductio n Transcriptio n regulator activity Transcripti on regulatory protein ARM; CC; No Yes gi5031595 ARPC4 NP_005709.1 Actin related protein 2/3 complex subunit 4 3 18.45 Cytoskeleton Cell growth and/or maintenanc e Cytoskeletal protein binding Cytoskelet al associated protein - Yes Yes gi5031597 ARPC3 NP_005710.1 ARP2/3 protein complex subunit p21 3 19.66 Cytoplasm Cell growth and/or maintenanc e Cytoskeletal protein binding Cytoskelet al associated protein - Yes Yes gi5031631 SCARB2 NP_005497.1 SCARB2 5 16.74 Plasma membrane Cell communica tion Signal transductio n Receptor activity Cell surface receptor TM; SP; Yes Yes gi5031635 CFL1 NP_005498.1 Cofilin 1 6 45.78 Cytoplasm Cell growth and/or maintenanc e Cytoskeletal protein binding Cytoskelet al associated protein ADF; NLS; Yes Yes gi5031695 CFHR2 NP_005657.1 Complement factor H related protein 2 4 17.04 Extracellular Immune response Molecular function unknown Secreted polypeptide SUSHI; SP; Yes Yes gi5031699 FLOT1 NP_005794.1 Flotillin 1 12 40.98 Plasma membrane Cell growth and/or maintenanc e Molecular function unknown Unclassifie d PHB; CC; Yes Yes gi5031719 GPC6 NP_005699.1 Glypican 6 2 5.77 Plasma membrane Biological_ process unknown Growth factor activity Growth factor SP; Yes Yes gi5031789 IMPA1 NP_005527.1 Myo-inositol monophosphatase 1 1 3.97 Cytoplasm Metabolism Energy pathways Phosphopro tein phosphatas e activity Enzyme: Phosphata se I_Phosphatas e; Yes Yes Protein accession Gene Symbol RefSeq accession Protein Description Unique number of peptides Sequence coverage Cellular component Biological process Molecular function Molecule class Architecture Replicate 1 Replicate 2 Supplemental Table 3. ..

    Migration:

    Article Title: Bone marrow mesenchymal stem cell-derived exosomes improve pyroptosis and mitochondrial integrity through miR-515-5p-mediated TLR4/NLRP3/GSDMD axis in rheumatoid arthritis
    Article Snippet: .. A Immunofluorescence analysis of RA-FLSs internalization of BMSCs-Exo; B Ach E activity assay to quantify BMSCs-Exo release; C – D Transwell assays to evaluate migration and invasion of RA-FLSs; E – F WB analysis showing representative images and quantification of ECM proteins collagen I and fibronectin; G Immunofluorescence staining of actin cytoskeleton in RA-FLSs. ..

    Article Title: Bone marrow mesenchymal stem cell-derived exosomes improve pyroptosis and mitochondrial integrity through miR-515-5p-mediated TLR4/NLRP3/GSDMD axis in rheumatoid arthritis.
    Article Snippet: .. A Immunofluorescence analysis of RA-FLSs internalization of BMSCs-Exo; B Ach E activity assay to quantify BMSCs-Exo release; C–D Transwell assays to evaluate migration and invasion of RA-FLSs; E–F WB analysis showing representative images and quantification of ECM proteins collagen I and fibronectin; G Immunofluorescence staining of actin cytoskeleton in RA-FLSs. ..

    Western Blot:

    Article Title: Bone marrow mesenchymal stem cell-derived exosomes improve pyroptosis and mitochondrial integrity through miR-515-5p-mediated TLR4/NLRP3/GSDMD axis in rheumatoid arthritis
    Article Snippet: .. A Immunofluorescence analysis of RA-FLSs internalization of BMSCs-Exo; B Ach E activity assay to quantify BMSCs-Exo release; C – D Transwell assays to evaluate migration and invasion of RA-FLSs; E – F WB analysis showing representative images and quantification of ECM proteins collagen I and fibronectin; G Immunofluorescence staining of actin cytoskeleton in RA-FLSs. ..

    Article Title: Bone marrow mesenchymal stem cell-derived exosomes improve pyroptosis and mitochondrial integrity through miR-515-5p-mediated TLR4/NLRP3/GSDMD axis in rheumatoid arthritis.
    Article Snippet: .. A Immunofluorescence analysis of RA-FLSs internalization of BMSCs-Exo; B Ach E activity assay to quantify BMSCs-Exo release; C–D Transwell assays to evaluate migration and invasion of RA-FLSs; E–F WB analysis showing representative images and quantification of ECM proteins collagen I and fibronectin; G Immunofluorescence staining of actin cytoskeleton in RA-FLSs. ..

    Staining:

    Article Title: Bone marrow mesenchymal stem cell-derived exosomes improve pyroptosis and mitochondrial integrity through miR-515-5p-mediated TLR4/NLRP3/GSDMD axis in rheumatoid arthritis
    Article Snippet: .. A Immunofluorescence analysis of RA-FLSs internalization of BMSCs-Exo; B Ach E activity assay to quantify BMSCs-Exo release; C – D Transwell assays to evaluate migration and invasion of RA-FLSs; E – F WB analysis showing representative images and quantification of ECM proteins collagen I and fibronectin; G Immunofluorescence staining of actin cytoskeleton in RA-FLSs. ..

    Article Title: Bone marrow mesenchymal stem cell-derived exosomes improve pyroptosis and mitochondrial integrity through miR-515-5p-mediated TLR4/NLRP3/GSDMD axis in rheumatoid arthritis.
    Article Snippet: .. A Immunofluorescence analysis of RA-FLSs internalization of BMSCs-Exo; B Ach E activity assay to quantify BMSCs-Exo release; C–D Transwell assays to evaluate migration and invasion of RA-FLSs; E–F WB analysis showing representative images and quantification of ECM proteins collagen I and fibronectin; G Immunofluorescence staining of actin cytoskeleton in RA-FLSs. ..

    Protein Binding:

    Article Title:
    Article Snippet: 1 ATP1B1 1 3.65 Plasma membrane Transport ATPase activity ATPase TM; Yes Yes gi49574534 HAVCR2 NP_116171.3 Hepatitis A virus cellular receptor 2 8 25.91 Plasma membrane Immune response Receptor activity Cell surface receptor IG; TM; SP; Yes Yes gi49574537 GCSH NP_004474.2 Glycine cleavage system H protein 1 11.56 Mitochondrion Metabolism Energy pathways Transferase activity Enzyme: Transferas e SP; Yes Yes gi50053795 EIF4B NP_001408.2 Eukaryotic translation initiation factor 4B 1 1.47 Cytoplasm Protein metabolism Translation regulator activity Translation regulatory protein RRM; CC; Yes Yes gi50234886 ZDHHC23 NP_775841.2 Zinc finger DHHC domain containing 23 1 2.45 Plasma membrane Cell communica tion Signal transductio n Molecular function unknown Unclassifie d TM; Yes No gi50301238 GSR NP_000628.2 Glutathione reductase 2 5.94 Mitochondrion Metabolism Energy pathways Catalytic activity Enzyme: Reductase SP; No Yes gi5031571 ACTR2 NP_005713.1 ARP2 actin-related protein 2 homolog 2 6.60 Actin cytoskeleton Cell motility Cell growth and/or maintenanc e Structural constituent of cytoskeleto n Cytoskelet al protein ACTIN; Yes Yes Protein accession Gene Symbol RefSeq accession Protein Description Unique number of peptides Sequence coverage Cellular component Biological process Molecular function Molecule class Architecture Replicate 1 Replicate 2 Supplemental Table 3. .. A Complete list of proteins identified from this study gi5031573 ACTR3 NP_005712.1 Actin related protein 3 5 20.57 Cytoplasm Cell growth and/or maintenanc e Structural constituent of cytoskeleto n Cytoskelet al protein ACTIN; Yes Yes gi5031587 APC2 NP_005874.1 Adenomatosis polyposis coli 2 1 0.26 Nucleus Cell communica tion Signal transductio n Transcriptio n regulator activity Transcripti on regulatory protein ARM; CC; No Yes gi5031595 ARPC4 NP_005709.1 Actin related protein 2/3 complex subunit 4 3 18.45 Cytoskeleton Cell growth and/or maintenanc e Cytoskeletal protein binding Cytoskelet al associated protein - Yes Yes gi5031597 ARPC3 NP_005710.1 ARP2/3 protein complex subunit p21 3 19.66 Cytoplasm Cell growth and/or maintenanc e Cytoskeletal protein binding Cytoskelet al associated protein - Yes Yes gi5031631 SCARB2 NP_005497.1 SCARB2 5 16.74 Plasma membrane Cell communica tion Signal transductio n Receptor activity Cell surface receptor TM; SP; Yes Yes gi5031635 CFL1 NP_005498.1 Cofilin 1 6 45.78 Cytoplasm Cell growth and/or maintenanc e Cytoskeletal protein binding Cytoskelet al associated protein ADF; NLS; Yes Yes gi5031695 CFHR2 NP_005657.1 Complement factor H related protein 2 4 17.04 Extracellular Immune response Molecular function unknown Secreted polypeptide SUSHI; SP; Yes Yes gi5031699 FLOT1 NP_005794.1 Flotillin 1 12 40.98 Plasma membrane Cell growth and/or maintenanc e Molecular function unknown Unclassifie d PHB; CC; Yes Yes gi5031719 GPC6 NP_005699.1 Glypican 6 2 5.77 Plasma membrane Biological_ process unknown Growth factor activity Growth factor SP; Yes Yes gi5031789 IMPA1 NP_005527.1 Myo-inositol monophosphatase 1 1 3.97 Cytoplasm Metabolism Energy pathways Phosphopro tein phosphatas e activity Enzyme: Phosphata se I_Phosphatas e; Yes Yes Protein accession Gene Symbol RefSeq accession Protein Description Unique number of peptides Sequence coverage Cellular component Biological process Molecular function Molecule class Architecture Replicate 1 Replicate 2 Supplemental Table 3. ..

    Clinical Proteomics:

    Article Title:
    Article Snippet: 1 ATP1B1 1 3.65 Plasma membrane Transport ATPase activity ATPase TM; Yes Yes gi49574534 HAVCR2 NP_116171.3 Hepatitis A virus cellular receptor 2 8 25.91 Plasma membrane Immune response Receptor activity Cell surface receptor IG; TM; SP; Yes Yes gi49574537 GCSH NP_004474.2 Glycine cleavage system H protein 1 11.56 Mitochondrion Metabolism Energy pathways Transferase activity Enzyme: Transferas e SP; Yes Yes gi50053795 EIF4B NP_001408.2 Eukaryotic translation initiation factor 4B 1 1.47 Cytoplasm Protein metabolism Translation regulator activity Translation regulatory protein RRM; CC; Yes Yes gi50234886 ZDHHC23 NP_775841.2 Zinc finger DHHC domain containing 23 1 2.45 Plasma membrane Cell communica tion Signal transductio n Molecular function unknown Unclassifie d TM; Yes No gi50301238 GSR NP_000628.2 Glutathione reductase 2 5.94 Mitochondrion Metabolism Energy pathways Catalytic activity Enzyme: Reductase SP; No Yes gi5031571 ACTR2 NP_005713.1 ARP2 actin-related protein 2 homolog 2 6.60 Actin cytoskeleton Cell motility Cell growth and/or maintenanc e Structural constituent of cytoskeleto n Cytoskelet al protein ACTIN; Yes Yes Protein accession Gene Symbol RefSeq accession Protein Description Unique number of peptides Sequence coverage Cellular component Biological process Molecular function Molecule class Architecture Replicate 1 Replicate 2 Supplemental Table 3. .. A Complete list of proteins identified from this study gi5031573 ACTR3 NP_005712.1 Actin related protein 3 5 20.57 Cytoplasm Cell growth and/or maintenanc e Structural constituent of cytoskeleto n Cytoskelet al protein ACTIN; Yes Yes gi5031587 APC2 NP_005874.1 Adenomatosis polyposis coli 2 1 0.26 Nucleus Cell communica tion Signal transductio n Transcriptio n regulator activity Transcripti on regulatory protein ARM; CC; No Yes gi5031595 ARPC4 NP_005709.1 Actin related protein 2/3 complex subunit 4 3 18.45 Cytoskeleton Cell growth and/or maintenanc e Cytoskeletal protein binding Cytoskelet al associated protein - Yes Yes gi5031597 ARPC3 NP_005710.1 ARP2/3 protein complex subunit p21 3 19.66 Cytoplasm Cell growth and/or maintenanc e Cytoskeletal protein binding Cytoskelet al associated protein - Yes Yes gi5031631 SCARB2 NP_005497.1 SCARB2 5 16.74 Plasma membrane Cell communica tion Signal transductio n Receptor activity Cell surface receptor TM; SP; Yes Yes gi5031635 CFL1 NP_005498.1 Cofilin 1 6 45.78 Cytoplasm Cell growth and/or maintenanc e Cytoskeletal protein binding Cytoskelet al associated protein ADF; NLS; Yes Yes gi5031695 CFHR2 NP_005657.1 Complement factor H related protein 2 4 17.04 Extracellular Immune response Molecular function unknown Secreted polypeptide SUSHI; SP; Yes Yes gi5031699 FLOT1 NP_005794.1 Flotillin 1 12 40.98 Plasma membrane Cell growth and/or maintenanc e Molecular function unknown Unclassifie d PHB; CC; Yes Yes gi5031719 GPC6 NP_005699.1 Glypican 6 2 5.77 Plasma membrane Biological_ process unknown Growth factor activity Growth factor SP; Yes Yes gi5031789 IMPA1 NP_005527.1 Myo-inositol monophosphatase 1 1 3.97 Cytoplasm Metabolism Energy pathways Phosphopro tein phosphatas e activity Enzyme: Phosphata se I_Phosphatas e; Yes Yes Protein accession Gene Symbol RefSeq accession Protein Description Unique number of peptides Sequence coverage Cellular component Biological process Molecular function Molecule class Architecture Replicate 1 Replicate 2 Supplemental Table 3. ..

    Membrane:

    Article Title:
    Article Snippet: 1 ATP1B1 1 3.65 Plasma membrane Transport ATPase activity ATPase TM; Yes Yes gi49574534 HAVCR2 NP_116171.3 Hepatitis A virus cellular receptor 2 8 25.91 Plasma membrane Immune response Receptor activity Cell surface receptor IG; TM; SP; Yes Yes gi49574537 GCSH NP_004474.2 Glycine cleavage system H protein 1 11.56 Mitochondrion Metabolism Energy pathways Transferase activity Enzyme: Transferas e SP; Yes Yes gi50053795 EIF4B NP_001408.2 Eukaryotic translation initiation factor 4B 1 1.47 Cytoplasm Protein metabolism Translation regulator activity Translation regulatory protein RRM; CC; Yes Yes gi50234886 ZDHHC23 NP_775841.2 Zinc finger DHHC domain containing 23 1 2.45 Plasma membrane Cell communica tion Signal transductio n Molecular function unknown Unclassifie d TM; Yes No gi50301238 GSR NP_000628.2 Glutathione reductase 2 5.94 Mitochondrion Metabolism Energy pathways Catalytic activity Enzyme: Reductase SP; No Yes gi5031571 ACTR2 NP_005713.1 ARP2 actin-related protein 2 homolog 2 6.60 Actin cytoskeleton Cell motility Cell growth and/or maintenanc e Structural constituent of cytoskeleto n Cytoskelet al protein ACTIN; Yes Yes Protein accession Gene Symbol RefSeq accession Protein Description Unique number of peptides Sequence coverage Cellular component Biological process Molecular function Molecule class Architecture Replicate 1 Replicate 2 Supplemental Table 3. .. A Complete list of proteins identified from this study gi5031573 ACTR3 NP_005712.1 Actin related protein 3 5 20.57 Cytoplasm Cell growth and/or maintenanc e Structural constituent of cytoskeleto n Cytoskelet al protein ACTIN; Yes Yes gi5031587 APC2 NP_005874.1 Adenomatosis polyposis coli 2 1 0.26 Nucleus Cell communica tion Signal transductio n Transcriptio n regulator activity Transcripti on regulatory protein ARM; CC; No Yes gi5031595 ARPC4 NP_005709.1 Actin related protein 2/3 complex subunit 4 3 18.45 Cytoskeleton Cell growth and/or maintenanc e Cytoskeletal protein binding Cytoskelet al associated protein - Yes Yes gi5031597 ARPC3 NP_005710.1 ARP2/3 protein complex subunit p21 3 19.66 Cytoplasm Cell growth and/or maintenanc e Cytoskeletal protein binding Cytoskelet al associated protein - Yes Yes gi5031631 SCARB2 NP_005497.1 SCARB2 5 16.74 Plasma membrane Cell communica tion Signal transductio n Receptor activity Cell surface receptor TM; SP; Yes Yes gi5031635 CFL1 NP_005498.1 Cofilin 1 6 45.78 Cytoplasm Cell growth and/or maintenanc e Cytoskeletal protein binding Cytoskelet al associated protein ADF; NLS; Yes Yes gi5031695 CFHR2 NP_005657.1 Complement factor H related protein 2 4 17.04 Extracellular Immune response Molecular function unknown Secreted polypeptide SUSHI; SP; Yes Yes gi5031699 FLOT1 NP_005794.1 Flotillin 1 12 40.98 Plasma membrane Cell growth and/or maintenanc e Molecular function unknown Unclassifie d PHB; CC; Yes Yes gi5031719 GPC6 NP_005699.1 Glypican 6 2 5.77 Plasma membrane Biological_ process unknown Growth factor activity Growth factor SP; Yes Yes gi5031789 IMPA1 NP_005527.1 Myo-inositol monophosphatase 1 1 3.97 Cytoplasm Metabolism Energy pathways Phosphopro tein phosphatas e activity Enzyme: Phosphata se I_Phosphatas e; Yes Yes Protein accession Gene Symbol RefSeq accession Protein Description Unique number of peptides Sequence coverage Cellular component Biological process Molecular function Molecule class Architecture Replicate 1 Replicate 2 Supplemental Table 3. ..

    Cell Surface Receptor Assay:

    Article Title:
    Article Snippet: 1 ATP1B1 1 3.65 Plasma membrane Transport ATPase activity ATPase TM; Yes Yes gi49574534 HAVCR2 NP_116171.3 Hepatitis A virus cellular receptor 2 8 25.91 Plasma membrane Immune response Receptor activity Cell surface receptor IG; TM; SP; Yes Yes gi49574537 GCSH NP_004474.2 Glycine cleavage system H protein 1 11.56 Mitochondrion Metabolism Energy pathways Transferase activity Enzyme: Transferas e SP; Yes Yes gi50053795 EIF4B NP_001408.2 Eukaryotic translation initiation factor 4B 1 1.47 Cytoplasm Protein metabolism Translation regulator activity Translation regulatory protein RRM; CC; Yes Yes gi50234886 ZDHHC23 NP_775841.2 Zinc finger DHHC domain containing 23 1 2.45 Plasma membrane Cell communica tion Signal transductio n Molecular function unknown Unclassifie d TM; Yes No gi50301238 GSR NP_000628.2 Glutathione reductase 2 5.94 Mitochondrion Metabolism Energy pathways Catalytic activity Enzyme: Reductase SP; No Yes gi5031571 ACTR2 NP_005713.1 ARP2 actin-related protein 2 homolog 2 6.60 Actin cytoskeleton Cell motility Cell growth and/or maintenanc e Structural constituent of cytoskeleto n Cytoskelet al protein ACTIN; Yes Yes Protein accession Gene Symbol RefSeq accession Protein Description Unique number of peptides Sequence coverage Cellular component Biological process Molecular function Molecule class Architecture Replicate 1 Replicate 2 Supplemental Table 3. .. A Complete list of proteins identified from this study gi5031573 ACTR3 NP_005712.1 Actin related protein 3 5 20.57 Cytoplasm Cell growth and/or maintenanc e Structural constituent of cytoskeleto n Cytoskelet al protein ACTIN; Yes Yes gi5031587 APC2 NP_005874.1 Adenomatosis polyposis coli 2 1 0.26 Nucleus Cell communica tion Signal transductio n Transcriptio n regulator activity Transcripti on regulatory protein ARM; CC; No Yes gi5031595 ARPC4 NP_005709.1 Actin related protein 2/3 complex subunit 4 3 18.45 Cytoskeleton Cell growth and/or maintenanc e Cytoskeletal protein binding Cytoskelet al associated protein - Yes Yes gi5031597 ARPC3 NP_005710.1 ARP2/3 protein complex subunit p21 3 19.66 Cytoplasm Cell growth and/or maintenanc e Cytoskeletal protein binding Cytoskelet al associated protein - Yes Yes gi5031631 SCARB2 NP_005497.1 SCARB2 5 16.74 Plasma membrane Cell communica tion Signal transductio n Receptor activity Cell surface receptor TM; SP; Yes Yes gi5031635 CFL1 NP_005498.1 Cofilin 1 6 45.78 Cytoplasm Cell growth and/or maintenanc e Cytoskeletal protein binding Cytoskelet al associated protein ADF; NLS; Yes Yes gi5031695 CFHR2 NP_005657.1 Complement factor H related protein 2 4 17.04 Extracellular Immune response Molecular function unknown Secreted polypeptide SUSHI; SP; Yes Yes gi5031699 FLOT1 NP_005794.1 Flotillin 1 12 40.98 Plasma membrane Cell growth and/or maintenanc e Molecular function unknown Unclassifie d PHB; CC; Yes Yes gi5031719 GPC6 NP_005699.1 Glypican 6 2 5.77 Plasma membrane Biological_ process unknown Growth factor activity Growth factor SP; Yes Yes gi5031789 IMPA1 NP_005527.1 Myo-inositol monophosphatase 1 1 3.97 Cytoplasm Metabolism Energy pathways Phosphopro tein phosphatas e activity Enzyme: Phosphata se I_Phosphatas e; Yes Yes Protein accession Gene Symbol RefSeq accession Protein Description Unique number of peptides Sequence coverage Cellular component Biological process Molecular function Molecule class Architecture Replicate 1 Replicate 2 Supplemental Table 3. ..

    Sequencing:

    Article Title:
    Article Snippet: 1 ATP1B1 1 3.65 Plasma membrane Transport ATPase activity ATPase TM; Yes Yes gi49574534 HAVCR2 NP_116171.3 Hepatitis A virus cellular receptor 2 8 25.91 Plasma membrane Immune response Receptor activity Cell surface receptor IG; TM; SP; Yes Yes gi49574537 GCSH NP_004474.2 Glycine cleavage system H protein 1 11.56 Mitochondrion Metabolism Energy pathways Transferase activity Enzyme: Transferas e SP; Yes Yes gi50053795 EIF4B NP_001408.2 Eukaryotic translation initiation factor 4B 1 1.47 Cytoplasm Protein metabolism Translation regulator activity Translation regulatory protein RRM; CC; Yes Yes gi50234886 ZDHHC23 NP_775841.2 Zinc finger DHHC domain containing 23 1 2.45 Plasma membrane Cell communica tion Signal transductio n Molecular function unknown Unclassifie d TM; Yes No gi50301238 GSR NP_000628.2 Glutathione reductase 2 5.94 Mitochondrion Metabolism Energy pathways Catalytic activity Enzyme: Reductase SP; No Yes gi5031571 ACTR2 NP_005713.1 ARP2 actin-related protein 2 homolog 2 6.60 Actin cytoskeleton Cell motility Cell growth and/or maintenanc e Structural constituent of cytoskeleto n Cytoskelet al protein ACTIN; Yes Yes Protein accession Gene Symbol RefSeq accession Protein Description Unique number of peptides Sequence coverage Cellular component Biological process Molecular function Molecule class Architecture Replicate 1 Replicate 2 Supplemental Table 3. .. A Complete list of proteins identified from this study gi5031573 ACTR3 NP_005712.1 Actin related protein 3 5 20.57 Cytoplasm Cell growth and/or maintenanc e Structural constituent of cytoskeleto n Cytoskelet al protein ACTIN; Yes Yes gi5031587 APC2 NP_005874.1 Adenomatosis polyposis coli 2 1 0.26 Nucleus Cell communica tion Signal transductio n Transcriptio n regulator activity Transcripti on regulatory protein ARM; CC; No Yes gi5031595 ARPC4 NP_005709.1 Actin related protein 2/3 complex subunit 4 3 18.45 Cytoskeleton Cell growth and/or maintenanc e Cytoskeletal protein binding Cytoskelet al associated protein - Yes Yes gi5031597 ARPC3 NP_005710.1 ARP2/3 protein complex subunit p21 3 19.66 Cytoplasm Cell growth and/or maintenanc e Cytoskeletal protein binding Cytoskelet al associated protein - Yes Yes gi5031631 SCARB2 NP_005497.1 SCARB2 5 16.74 Plasma membrane Cell communica tion Signal transductio n Receptor activity Cell surface receptor TM; SP; Yes Yes gi5031635 CFL1 NP_005498.1 Cofilin 1 6 45.78 Cytoplasm Cell growth and/or maintenanc e Cytoskeletal protein binding Cytoskelet al associated protein ADF; NLS; Yes Yes gi5031695 CFHR2 NP_005657.1 Complement factor H related protein 2 4 17.04 Extracellular Immune response Molecular function unknown Secreted polypeptide SUSHI; SP; Yes Yes gi5031699 FLOT1 NP_005794.1 Flotillin 1 12 40.98 Plasma membrane Cell growth and/or maintenanc e Molecular function unknown Unclassifie d PHB; CC; Yes Yes gi5031719 GPC6 NP_005699.1 Glypican 6 2 5.77 Plasma membrane Biological_ process unknown Growth factor activity Growth factor SP; Yes Yes gi5031789 IMPA1 NP_005527.1 Myo-inositol monophosphatase 1 1 3.97 Cytoplasm Metabolism Energy pathways Phosphopro tein phosphatas e activity Enzyme: Phosphata se I_Phosphatas e; Yes Yes Protein accession Gene Symbol RefSeq accession Protein Description Unique number of peptides Sequence coverage Cellular component Biological process Molecular function Molecule class Architecture Replicate 1 Replicate 2 Supplemental Table 3. ..



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    Image Search Results


    a , Immunoblots showing cleaved Notch1 (c-Notch1) and total Notch1 in MVECs treated with S1P for 1-hour ± γ-secretase inhibitor DAPT; quantification below (mean ± SEM). b , Fluorescent micrographs of YFP-Notch1 reporter intensity after S1P treatment; quantification below. Scale bar, 50 μm. c-d , Fold change in HES1 and HEY1 gene expression levels in (c) MVECs after S1P ± DAPT treatment, and in (d) mouse tissues after intravenous injection of S1P ± DAPT. e , Evans blue dye leakage in mouse dermis 1-hour post-injection with DMSO, S1P, or S1P + DAPT. Scale bar, 5 mm. f-i , Quantified dye leakage in (f) dermis, (g) lung, (h) liver, and (i) kidney, measured via absorbance. j , Heat maps of dextran dye diffusion in engineered microvessels ± S1P. Scale bar, 50 μm. k-l , Diffusive permeability in microvessels lined with (k) DAPT-vs. DMSO-treated or (l) NOTCH1 KO vs. SCR KO MVECs (mean ± SD). m , Micrographs of VE-cadherin (magenta), actin (green), and nuclei (blue) staining in microvessels. Scale bar, 50 μm. n-o , Mean corrected intensities of junctional (n) VE-cadherin and (o) actin from (m). p , Rac1 activity (PBD pull-down). Active/total Rac1 quantification below. q , Schematic of canonical Notch signaling. r , HES1 / HEY1 gene expression levels in dominant negative-MAML-GFP (DN-MAML-GFP) normalized to GFP expressing cells. s , Permeability in DN-MAML-GFP vs GFP-lined microvessels ± S1P. t , Schematic of Notch cortical pathway mediated by its transmembrane domain (TMD). u , Permeability in TMD-RFP vs RFP-lined microvessels ± S1P. v , Permeability in TMD-RFP vs RFP-lined microvessels ± clinical S1P receptor degrader fingolimod.

    Journal: bioRxiv

    Article Title: Sphingosine-1-phosphate cross-talks to Notch via a S1PR1-Dll4-MPDZ complex to regulate endothelial barrier function

    doi: 10.64898/2026.05.20.726610

    Figure Lengend Snippet: a , Immunoblots showing cleaved Notch1 (c-Notch1) and total Notch1 in MVECs treated with S1P for 1-hour ± γ-secretase inhibitor DAPT; quantification below (mean ± SEM). b , Fluorescent micrographs of YFP-Notch1 reporter intensity after S1P treatment; quantification below. Scale bar, 50 μm. c-d , Fold change in HES1 and HEY1 gene expression levels in (c) MVECs after S1P ± DAPT treatment, and in (d) mouse tissues after intravenous injection of S1P ± DAPT. e , Evans blue dye leakage in mouse dermis 1-hour post-injection with DMSO, S1P, or S1P + DAPT. Scale bar, 5 mm. f-i , Quantified dye leakage in (f) dermis, (g) lung, (h) liver, and (i) kidney, measured via absorbance. j , Heat maps of dextran dye diffusion in engineered microvessels ± S1P. Scale bar, 50 μm. k-l , Diffusive permeability in microvessels lined with (k) DAPT-vs. DMSO-treated or (l) NOTCH1 KO vs. SCR KO MVECs (mean ± SD). m , Micrographs of VE-cadherin (magenta), actin (green), and nuclei (blue) staining in microvessels. Scale bar, 50 μm. n-o , Mean corrected intensities of junctional (n) VE-cadherin and (o) actin from (m). p , Rac1 activity (PBD pull-down). Active/total Rac1 quantification below. q , Schematic of canonical Notch signaling. r , HES1 / HEY1 gene expression levels in dominant negative-MAML-GFP (DN-MAML-GFP) normalized to GFP expressing cells. s , Permeability in DN-MAML-GFP vs GFP-lined microvessels ± S1P. t , Schematic of Notch cortical pathway mediated by its transmembrane domain (TMD). u , Permeability in TMD-RFP vs RFP-lined microvessels ± S1P. v , Permeability in TMD-RFP vs RFP-lined microvessels ± clinical S1P receptor degrader fingolimod.

    Article Snippet: PAK-PBD beads to bind active Rac1 (PAK-02) were purchased from Cytoskeleton and reconstituted according to the manufacturer’s instructions.

    Techniques: Western Blot, Gene Expression, Injection, Diffusion-based Assay, Permeability, Staining, Activity Assay, Dominant Negative Mutation, Expressing